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* For the value, provide the median coverage expected in autosomal regions with coverage. The mode accepts only a
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* single sample, which can be provided in multiple files.</p>
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*
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+ * <h4>(iv) Force-calling mode</h4>
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+ * <p>This mode force-calls all alleles in force-call-alleles.vcf in addition to any other variants Mutect2 discovers.
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+ *
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+ * <pre>
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+ * gatk Mutect2 \
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+ * -R reference.fa \
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+ * -I sample.bam \
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+ * -alleles force-call-alleles.vcf
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+ * -tumor sample_name \
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+ * -O single_sample.vcf.gz
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+ * </pre>
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+ *
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* <h3>Notes</h3>
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* <ol>
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* <li>Mutect2 does not require a germline resource nor a panel of normals (PoN) to run. The tool prefilters sites
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